Stephanie Ramadan
Assistant Professor
LS-403
Human Biology
Research Interests
Dr. Ramadan's research focuses on elucidating protein-protein interactions across various model organisms, including, most recently, humans and Helicobacter pylori. These interactions are fundamental to numerous biological processes, and identifying new ones has the potential to enhance our molecular understanding and pave the way for therapeutic advances. Over the past decade, Dr. Ramadan, in collaboration with her research team at Weill Cornell Medicine in Qatar, developed and refined a powerful method known as AVA-Seq (All vs All Sequencing). This innovative approach combines next-generation sequencing with principles from yeast and bacterial two-hybrid systems to comprehensively map protein interactions. The vast dataset generated from AVA-Seq is now being leveraged for in vitro validation using classical biochemistry and molecular biology techniques, further advancing this groundbreaking work.
If you are an undergraduate at UWGB and interested in joining Dr. Ramadan’s research ambitions, please send her an email.
Education
B.S. in Chemistry – Marian University - 2007
Ph.D. in Chemistry and Biochemistry – University of Delaware - 2013
MBA in Entrepreneurship – Johns Hopkins Carey Business School – 2021
Select Publications
Dr. Ramadan's research focuses on elucidating protein-protein interactions across various model organisms, including, most recently, humans and Helicobacter pylori. These interactions are fundamental to numerous biological processes, and identifying new ones has the potential to enhance our molecular understanding and pave the way for therapeutic advances. Over the past decade, Dr. Ramadan, in collaboration with her research team at Weill Cornell Medicine in Qatar, developed and refined a powerful method known as AVA-Seq (All vs All Sequencing). This innovative approach combines next-generation sequencing with principles from yeast and bacterial two-hybrid systems to comprehensively map protein interactions. The vast dataset generated from AVA-Seq is now being leveraged for in vitro validation using classical biochemistry and molecular biology techniques, further advancing this groundbreaking work.
If you are an undergraduate at UWGB and interested in joining Dr. Ramadan’s research ambitions, please send her an email.
Education
B.S. in Chemistry – Marian University - 2007
Ph.D. in Chemistry and Biochemistry – University of Delaware - 2013
MBA in Entrepreneurship – Johns Hopkins Carey Business School – 2021
Select Publications
- Al-Thani N.M., Schaefer-Ramadan S., Aleksic J., Mohamoud Y.A. and Malek J.A. Identifying novel interactions of the colon-cancer related APC protein with Wnt-pathway nuclear transcription factors. Cancer Cell Int. 2022.
- Schaefer-Ramadan S., Aleksic J., Al-Thani NM and Malek J.A. Novel protein contact points among TP53 and minichromosome maintenance complex proteins 2, 3 and 5. Cancer Med. 2022.
- Schaefer-Ramadan S., Aleksic J., Al-Thani N.M., Mohamoud Y.A., Hill DE and Malek J.A. Scaling-up a fragment-based protein-protein interaction method using a human reference set. Proteins. 2022.
- *Schaefer-Ramadan S., *Andrews S.S., Al-Thani N.M., Ahmed I., Mohamoud Y.A. and Malek J.A. High resolution protein-protein interaction mapping using all-versus-all sequencing (AVA-seq). J Biol Chem. 2019. *contributed equally.
- Schaefer-Ramadan S., Barlog M., Roach J., Al-Hashimi M., Bazzi H.S. and Machaca K. Synthesis of TPEN variants to improve cancer cells selective killing capacity. Bioorg Chem. 2019.
- Schaefer-Ramadan S., Hubrack, S., and Machaca, K. Transition metal dependent regulation of the signal transduction cascade driving oocyte meiosis. J Cell Physiol. 2018.
- Schaefer-Ramadan S., Thorpe C. and Rozovsky S. Site-specific insertion of selenium into the redox-active disulfide of the flavoprotein Augmenter of Liver Regeneration. Arch Biochem Biophys. 2014.
- Schaefer-Ramadan S., Gannon S. and Thorpe C. Human Augmenter of Liver Regeneration: Probing the Catalytic Mechanism of a Flavin-Dependent Sulfhydryl Oxidase. Biochemistry. 2013.
- Schaefer S.A., Dong M., Rubenstein R., Wilkie W., Bahnson B.J., Thorpe C. and Rozovksy S. 77Se enrichment of proteins expands the biological NMR toolbox. J Mol Biol. 2013.
- Daithankar V.N., Schaefer S.A., Dong M., Bahnson B.J. and Thorpe C. Structure of the human sulfhydryl oxidase augmenter of liver regeneration and characterization of a human mutation causing an autosomal recessive myopathy. Biochem. 2010.
- Trevino S.R., Schaefer S.A., Scholtz J.M. and Pace CN Increasing protein conformational stability by optimizing beta-turn sequence, J Mol Biol. 2007.